Research Archive

Theoretical upper bound of multiplexing in biological sensory receptors

Through a Langevin dynamics simulation of a ligand-receptor sensor in a bath of ligands, we demonstrate that a binary-state receptor can simultaneously encode multiple independent environmental variables, such as ligand concentration and the speed of media flow.

Versatile Triphenylphosphine-Containing Polymeric Catalysts and Elucidation of Structure–Function Relationships

We synthesized a panel of polymeric catalysts containing a novel triphenylphosphine acrylamide monomer and investigated how their properties impact the rate of a model Suzuki–Miyaura cross-coupling reaction.

Using NMR-detected hydrogen-deuterium exchange to quantify protein stability in cosolutes, under crowded conditions in vitro and in cells

We review the use of nuclear magnetic resonance (NMR) spectroscopy to assess the exchange of amide protons for deuterons (HDX) in efforts to understand how high concentration of cosolutes, especially macromolecules, affect the equilibrium thermodynamics of protein stability.

Investigating Fundamental Principles of Nonequilibrium Assembly Using Temperature-Sensitive Copolymers

Using a model system of poly(ethylene glycol) methacrylate-based thermoresponsive lower critical solution temperature (LCST) copolymers, we explored the impact of composition and stimulus path on nonequilibrium thermal hysteretic behavior.

Single-Molecule Correlated Chemical Probing: A Revolution in RNA Structure Analysis

Enabled by a specialized reverse transcription strategy called mutational profiling (MaP), multiple chemical modification events can be measured within individual RNA molecules.

In Situ Attenuated Total Reflectance Infrared Spectroelectrochemistry (ATR-IR-SEC) for the Characterization of Molecular Redox Processes on Surface-Proximal Doped Silicon ATR Crystal Working Electrodes

Here, we have developed a new strategy to use multiple-reflection attenuated total reflectance IR spectroscopy (ATR-IR) coupled with electrochemistry to characterize catalysts directly integrated with a semiconductor surface under applied potential.

Innovations in targeting RNA by fragment-based ligand discovery

Here we share an integrated analysis of recent innovations in FBLD, emphasizing opportunities resulting from fragment elaboration via both linking and growing.

Bile salt hydrolases shape the bile acid landscape and restrict Clostridioides difficile growth in the murine gut

Here we survey the diversity of BSHs in the gut commensals Lactobacillaceae, which are commonly used as probiotics, and other members of the human gut microbiome.

Macromolecular Crowding by Polyethylene Glycol Reduces Protein Breathing

We used NMR to assess the effects of ethylene glycol (EG) and polyethylene glycols (PEGs) on the structure and stability of the B1 domain of protein G (GB1).

Salt Effect on the Viscosity of Semidilute Polyelectrolyte Solutions: Sodium Polystyrenesulfonate

We studied the viscosity of semidilute aqueous solutions of sodium polystyrenesulfonate as a function of polymer and salt concentrations.

Deformation Driven Deswelling of Brush Gels

We studied equilibrium swelling in brush gels undergoing large uniaxial deformations in contact with solvent by using a combination of the Flory–Rehner and scaling models of gels and coarse-grained molecular dynamics simulations of swollen brush networks.

Oligo(ethylene glycol) Side Chain Architecture Enables Alcohol-Processable Conjugated Polymers for Organic Solar Cells

In this work, we report the synthesis and characterization of three conjugated polymers derived from poly[(thiophene)-alt-(6,7-difluoro-2-(2-hexyldecyloxy)quinoxaline)] (PTQ10) with the goal of developing derivates which are more green-solvent-processable.