Research Archive

Photoactivatable Reporter to Perform Multiplexed and Temporally Controlled Measurements of Kinase and Protease Activity in Single Cells

Peptide bioreporters were developed to perform multiplexed measurements of the activation of epidermal growth factor receptor kinase (EGFR), Akt kinase (Akt/protein kinase B), and proteases/peptidases in single cells.

Effects of Ligand Shell Composition on Surface Reduction in PbS Quantum Dots

In this work, we evaluated the impacts of both surface-binding head group and ligand backbone on the properties and reactivity of PbS QDs through a partial exchange of native oleate ligands with undec-10-enoic acid, p-toluate, and undec-10-ene-1-thiol to access mixed-shell QDs.

Enabling practical nanoparticle electrodeposition from aqueous nanodroplets

Historically, the cost of nanodroplet-mediated electrodeposition experimentation is prohibitive because practitioners use 0.1 M to 0.5 M tetrabutylammonium perchlorate (TBAP) dissolved in the oil phase (∼10 mL). Here, we show that supporting electrolyte is not necessary for the oil phase.

A Semiconductor-Mediator-Catalyst Artificial Photosynthetic System for Photoelectrochemical Water Oxidation

In fabricating an artificial photosynthesis (AP) electrode for water oxidation, we have devised a semiconductor-mediator-catalyst structure that mimics photosystem II (PSII).

Enzymatic Synthesis of Diverse Heterocycles by a Noncanonical Nonribosomal Peptide Synthetase

Here, we characterize a monomodular NRPS, PvfC, encoded by the Pseudomonas virulence factor (pvf) gene cluster that is essential for virulence and signaling in different bacterial species.

Mechanisms of Electrochemical N2 Splitting by a Molybdenum Pincer Complex

The viability of electrochemical N2 binding and splitting by a molybdenum(III) pincer complex, (pyPNP)MoBr3 (pyPNP = 2,6-bis(tBu2PCH2)-C5H3N)), is established in this work, providing a foundation for a detailed mechanistic study of electrode-driven formation of the nitride complex (pyPNP)Mo(N)Br.

Subsite Ligand Recognition and Cooperativity in the TPP Riboswitch: Implications for Fragment-Linking in RNA Ligand Discovery

Here, we investigated the binding of thiamine and methylene diphosphonic acid (MDP, a soluble structural analogue of pyrophosphate) to the thiamine pyrophosphate riboswitch.

Divergent Reactivity of α,α-Disubstituted Alkenyl Hydrazones: Bench Stable Cyclopropylcarbinyl Equivalents

Herein we report the divergent reactivity of 2,2-dialkyl-3-(E)-alkenyl N-tosylhydrazones using Pd-catalyzed cross-coupling conditions, which enable the Z-selective synthesis of 3-aryl-1,4-dienes and gem-dialkyl vinylcyclopropanes.

Systematic Variation of Both the Aromatic Cage and Dialkyllysine via GCE-SAR Reveal Mechanistic Insights in CBX5 Reader Protein Binding

This study describes the development of a two-pronged approach combining genetic code expansion (GCE) and structure–activity relationships (SAR) through systematic variation of both the aromatic binding pocket in the protein and the alkyllysine residues in the peptide to probe inhibitor recognition in the CBX5 chromodomain.

Bio-inspired spectropolarimetric sensor based on tandem organic photodetectors and multi-twist liquid crystals

Here, we demonstrate a bio-inspired spectral and polarization sensor structure based on integrating semitransparent polarization-sensitive organic photovoltaics (P-OPVs) and liquid crystal polymer (LCP) retarders in a tandem configuration.

Amaranthus hypochondriacus seeds as a rich source of cysteine rich bioactive peptides

Here, in silico predictions, proteomics, and simulated gastrointestinal digestions are leveraged to identify digestion resistant CRPs within A. hypochondriacus seeds.

Mapping the Morphological Landscape of Oligomeric Di-block Peptide-Polymer Amphiphiles

We herein present a systematic study of PPA structure-assembly relationships.