Research Archive

Structure and Function of a Dehydrating Condensation Domain in Nonribosomal Peptide Biosynthesis

Here, we provide biochemical and bioinformatic evidence in support of the role of a unique class of condensation domains in dehydration (CmodAA).

Metagenomics combined with activity-based proteomics point to gut bacterial enzymes that reactivate mycophenolate

Here, we compare the fecal microbiomes of transplant recipients receiving MMF to healthy individuals using shotgun metagenomic sequencing.

HepaRG Cells Adopt Zonal-Like Drug-Metabolizing Phenotypes under Physiologically Relevant Oxygen Tensions and Wnt/beta-Catenin Signaling

We hypothesized that incorporating physiologically relevant environments would promote post-differentiation patterning of hepatocytes and result in zonal-like characteristics. To test this hypothesis, we evaluated the transcriptional regulation and activity of drug-metabolizing enzymes in HepaRG cells exposed to three different oxygen tensions, in the presence or absence of Wnt/β-catenin signaling.

Aliphatic C-H Functionalization Using Pyridine N-Oxides as H-Atom Abstraction Agents

The alkylation and heteroarylation of unactivated tertiary, secondary, and primary C(sp3)–H bonds was achieved by employing an acridinium photoredox catalyst along with readily available pyridine N-oxides as hydrogen atom transfer (HAT) precursors under visible light.

HFIP in Organic Synthesis

In this review, following a brief history of HFIP in organic synthesis and an overview of its physical properties, literature examples of organic reactions using HFIP as a solvent or an additive are presented, emphasizing the effect of solvent of each reaction.

A dual initiator approach for oxygen tolerant RAFT polymerization

Herein we report a broadly applicable approach to “polymerize through” oxygen using the synergistic combination of two radical initiators having different rates of homolysis.

Initial pH Governs Secondary Organic Aerosol Phase State and Morphology after Uptake of Isoprene Epoxydiols (IEPDX)

Herein, we show distinct differences in the morphology, phase state, and chemical composition of individual organic–inorganic mixed particles after IEPOX uptake to ammonium sulfate particles with different initial atmospherically relevant acidities (pH = 1, 3, and 5).

A structural metagenomics pipeline for examining the gut microbiome

Here, we outline a pipeline that combines microbial whole genome sequencing with protein structure data to yield a structural metagenomics-informed atlas of microbial enzyme families of interest.

All-Electron BSE@GW Method for K-Edge Core Electron Excitation Energies

We present an accurate computational approach to calculate absolute K-edge core electron excitation energies as measured by X-ray absorption spectroscopy.

RNA-Ligand Interactions Quantified by Surface Plasmon Resonance with Reference Subtraction

Here, we show that referencing to a channel containing a non-binding control RNA enables subtraction of nonspecific binding contributions, allowing measurements of accurate and specific binding affinities.

Quantitative prediction of variant effects on alternative splicing in MAPT using endogenous pre-messenger RNA structure probing

Here we use a novel chemical probing strategy to visualize endogenous precursor and mature MAPT mRNA structures in cells.

Application of an Imprint-and-Report Sensor Array for Detection of the Dietary Metabolite Trimethylamine N-Oxide and Its Precursors in Complex Mixtures

Thus, we have adapted our previously described "imprint-and-report" fluorescent sensing method using dynamic combinatorial libraries (DCLs) to create a sensor array for these four metabolites that functions at physiologically relevant concentrations.