This review details the progress toward the electroanalytical detection and evaluation of single enzyme kinetics largely focused on the nanoimpact method, a chronoamperometric detection strategy that monitors the change in the current-time profile associated with stochastic collisions of freely diffusing entities (e.g., enzymes) onto a microelectrode or nanoelectrode surface.
This study highlights the utility of plant defensin γ-core motif synthetic analogs for characterization of novel defensin activity.
One exciting subset of single-entity electrochemistry, and the topic of this review, is the study of reactions in nanoreactors of subfemtoliter (10−15 L) volumes with a particular focus on nanoparticle synthesis.
Here, we report the use of pincer-ligated iridium complexes to achieve alkane dehydrogenation by proton-coupled electron transfer, using pairs of oxidants and bases as proton and electron acceptors.
In this study, we quantified an increasing concentration response of three well-established α-pinene SOA tracers (pinic, pinonic, and 3-methyl-1,2,3-butanetricarboxylic acids) and a full mixture of α-pinene SOA in A549 (alveolar epithelial carcinoma) and BEAS-2B (bronchial epithelial normal) lung cell lines.
Herein, we demonstrate the β-functionalization of saturated aza-heterocycles enabled by a two-step organic photoredox catalysis approach.
Here, we demonstrate how to obtain these parameters from the concentration dependence of solution viscosity. The centerpiece of this approach is the scaling relationship between solution correlation length (blob size) ξ = lgν/B and the number of monomers per correlation blob g for polymers with monomer projection length l.
We review quantitative, wet-experiment based efforts aimed at understanding how and why high concentrations of small molecules, synthetic polymers, biologically relevant cosolutes and the interior of living cells affect the energetics of protein-protein interactions.
Here, we show that phosphorus (P) regulates TORC1 signaling in the model green alga Chlamydomonas (Chlamydomonas reinhardtii) via LST8, a conserved TORC1 subunit that interacts with the kinase domain of TOR.
We describe the observation and quantification of immiscible solvent entrapment on a carbonaceous electrode surface using microscopy-coupled electrogenerated chemiluminescence (ECL).