Transwell Assays

Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In work published in the journal Analyst, researchers in the Lockett Lab describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix.

The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, the group members quantify invasion: upon addition of an exogenous factor or in the presence of medium obtained from other cell types, in an indirect or direct co-culture format whose medium composition is dynamically changing, and in a single-zone or parallel, 96-zone, format.